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Indian Hedgehog antibody (C-Term)

This Rabbit Polyclonal antibody specifically detects Indian Hedgehog in WB and EIA. It exhibits reactivity toward Human and Mouse.
Catalog No. ABIN357829

Quick Overview for Indian Hedgehog antibody (C-Term) (ABIN357829)

Target

See all Indian Hedgehog (IHH) Antibodies
Indian Hedgehog (IHH)

Reactivity

  • 66
  • 24
  • 21
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human, Mouse

Host

  • 78
  • 6
  • 4
Rabbit

Clonality

  • 77
  • 11
Polyclonal

Conjugate

  • 42
  • 9
  • 7
  • 6
  • 4
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Indian Hedgehog antibody is un-conjugated

Application

  • 58
  • 36
  • 36
  • 16
  • 13
  • 13
  • 12
  • 10
  • 3
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Enzyme Immunoassay (EIA)
  • Binding Specificity

    • 15
    • 11
    • 8
    • 8
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    Specificity

    This antibody detects Ihh (C-term). Predicted to cross react with Zebrafish.

    Purification

    Protein A Chromatography followed by peptide affinity purification.

    Immunogen

    This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of mouse IHH.

    Isotype

    Ig Fraction
  • Application Notes

    ELISA: 1/1,000. Western blot: 1/50-1/100.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.25 mg/mL

    Buffer

    PBS with 0.09 % (W/V) Sodium Azide as preservative.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    4 °C/-20 °C

    Storage Comment

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • Target

    Indian Hedgehog (IHH)

    Alternative Name

    Indian Hedgehog Protein / IHH

    Background

    Ihh (Indian hedgehog) is a critical and possibly direct regulator of joint development. In its absence, distribution and function of Gdf5-expressing interzone-associated cells are abnormal.Synonyms: HHG-2, HHG2

    Molecular Weight

    49445 Da

    Gene ID

    16147

    NCBI Accession

    NP_034674

    UniProt

    P97812

    Pathways

    Hedgehog Signaling
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