SMAD4 antibody (pThr277)

Catalog No. ABIN358227

The Rabbit Polyclonal anti-SMAD4 antibody (ABIN358227) specifically detects SMAD4 in IHC (p) and EIA. The antibody is reactive with Human samples.

Quick Overview for SMAD4 antibody (pThr277) (ABIN358227)

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD4 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-SMAD4 Antibody

Binding Specificity: pThr277

Specificity: This antibody detects SMAD4 pThr277.

Cross-Reactivity (Details): Species reactivity (expected):Mouse (100 % Antigen Homology).
Species reactivity (tested):Human.

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T277 of human SMAD4.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Dot Blot: 1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Alternative Name: SMAD4

Background: Common mediator of signal transduction by TGF-beta (transforming growth factor) superfamily, SMAD4 is the common SMAD (co-SMAD). It promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. It may act as a tumor suppressor.Synonyms: DPC4, Deletion target in pancreatic carcinoma 4, MAD homolog 4, MADH4, Mothers against DPP homolog 4, Mothers against decapentaplegic homolog 4, SMAD 4, SMAD family member 4, SMAD-4

Molecular Weight: 60439 Da

Gene ID: 4089, 9606

UniProt: Q13485

Pathways: Cell Division Cycle, Chromatin Binding, Autophagy