SPPL2A antibody (N-Term)

Catalog No. ABIN358852

This Rabbit Polyclonal antibody specifically detects SPPL2A in WB, IHC (p) and EIA. It exhibits reactivity toward Human.

Quick Overview for SPPL2A antibody (N-Term) (ABIN358852)

Target: SPPL2A (Signal Peptide Peptidase-Like 2A (SPPL2A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPPL2A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-SPPL2A Antibody

Binding Specificity: N-Term

Specificity: This antibody reacts to SPPL2a.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human SPPL2a.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/10 - 1/50. Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for SPPL2A

Target: SPPL2A (Signal Peptide Peptidase-Like 2A (SPPL2A))

Alternative Name: SPPL2A

Background: By searching sequence databases for homologs of Dictyostelium discoideum Impas, which shares homology with presenilin, followed by PCR of lymphocyte and hippocampus cDNA libraries, Grigorenko et al. (2002) cloned SPPL2a. This protein, which may act as an intramembrane protease, contains an N-terminal protease-associated (PA) domain, several transmembrane regions, a hydrophilic loop, conservative sequences around the first and second aspartate residues, and an invariant PAL motif near the C terminus.Synonyms: IMP-3, IMP3, Intramembrane protease 3, PSL2, Presenilin-like protein 2, SPP-like 2A, Signal peptide peptidase-like 2A

Gene ID: 84888, 9606

UniProt: Q8TCT8