ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/10 - 1/50. Flow cytometry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Concentration
0.25 mg/mL
Buffer
PBS with 0.09 % (W/V) sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
RGD1563001 antibody, SPPL2A antibody, LOC453430 antibody, LOC100231183 antibody, IMP3 antibody, PSL2 antibody, 2010106G01Rik antibody, C130089K23Rik antibody, signal peptide peptidase-like 2A antibody, signal peptide peptidase like 2A antibody, Signal peptide peptidase-like 2A antibody, Sppl2a antibody, SPPL2A antibody, sppl2a antibody, psl2 antibody
Background
By searching sequence databases for homologs of Dictyostelium discoideum Impas, which shares homology with presenilin, followed by PCR of lymphocyte and hippocampus cDNA libraries, Grigorenko et al. (2002) cloned SPPL2a. This protein, which may act as an intramembrane protease, contains an N-terminal protease-associated (PA) domain, several transmembrane regions, a hydrophilic loop, conservative sequences around the first and second aspartate residues, and an invariant PAL motif near the C terminus.Synonyms: IMP-3, IMP3, Intramembrane protease 3, PSL2, Presenilin-like protein 2, SPP-like 2A, Signal peptide peptidase-like 2A