PKLR antibody (N-Term)

Catalog No. ABIN359109

This anti-PKLR antibody is a Rabbit Polyclonal antibody detecting PKLR in WB, IHC (p) and EIA. Suitable for Human and Mouse.

Quick Overview for PKLR antibody (N-Term) (ABIN359109)

Target: PKLR (Pyruvate Kinase, Liver and RBC (PKLR))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKLR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-PKLR Antibody

Binding Specificity: N-Term

Specificity: This antibody reacts to PKLR.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human PKLR.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for PKLR

Target: PKLR (Pyruvate Kinase, Liver and RBC (PKLR))

Alternative Name: PKLR

Background: PKLR is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA).Synonyms: PK1, PKL, Pyruvate kinase 1, Pyruvate kinase R/L, Pyruvate kinase isozymes R/L, R-type/L-type pyruvate kinase, Red cell/liver pyruvate kinase

Gene ID: 5313, 9606

UniProt: P30613

Pathways: Ribonucleoside Biosynthetic Process