SPHK2 antibody (C-Term)

Catalog No. ABIN359441

Product Details anti-SPHK2 Antibody

Target: SPHK2 (Sphingosine Kinase 2 (SPHK2))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPHK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human SPHK2.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for SPHK2

Target: SPHK2 (Sphingosine Kinase 2 (SPHK2))

Alternative Name: Sphingosine Kinase 2 / SPK2 (SPHK2 Products)

Synonyms: CG2159 antibody, CG32484 antibody, Dmel\\CG32484 antibody, SK2 antibody, Sphk2 antibody, sk2 antibody, DKFZp469F224 antibody, SK 2 antibody, SK-2 antibody, SPK 2 antibody, SPK-2 antibody, C76851 antibody, Sphingosine kinase antibody, sphk2 antibody, Sphingosine kinase 2 antibody, sphingosine kinase 2 antibody, sphK2 antibody, Sk2 antibody, SPHK2 antibody, Sphk2 antibody

Background: Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.Synonyms: SK2, SPHK2

Molecular Weight: 69217 Da

Gene ID: 56848, 9606

UniProt: Q9NRA0

Pathways: VEGF Signaling