PRKAA1 antibody (Ser487)

Catalog No. ABIN359481

This anti-PRKAA1 antibody is a Rabbit Polyclonal antibody detecting PRKAA1 in WB and IHC (p). Suitable for Human.

Quick Overview for PRKAA1 antibody (Ser487) (ABIN359481)

Target: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKAA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PRKAA1 Antibody

Binding Specificity: Ser487

Specificity: This antibody detects AMPK alpha (PRKAA1).

Purification: Protein A column, followed by peptide affinity purification.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S487 of human PRKAA1.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA 1: 1,000. Western blot 1: 50 - 1: 100. Immunohistochemistry 1: 10 - 1: 50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for PRKAA1

Target: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Alternative Name: PRKAA1

Background: PRKAA1 belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.Synonyms: 5'-AMP-activated protein kinase catalytic subunit alpha-1, AMPK alpha-1 chain, AMPK1

Molecular Weight: 63878 Da

Gene ID: 5562, 9606

UniProt: Q13131

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect