Isocitrate Dehydrogenase antibody (Middle Region)

Catalog No. ABIN359618

The Rabbit Polyclonal anti-Isocitrate Dehydrogenase antibody is suitable to detect Isocitrate Dehydrogenase in samples from Human. It has been validated for WB and EIA.

Quick Overview for Isocitrate Dehydrogenase antibody (Middle Region) (ABIN359618)

Target: Isocitrate Dehydrogenase (IDH)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Isocitrate Dehydrogenase antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Isocitrate Dehydrogenase Antibody

Binding Specificity: Middle Region

Specificity: This antibody reacts to IDH1.

Purification: Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human IDH1.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for Isocitrate Dehydrogenase

Target: Isocitrate Dehydrogenase (IDH)

Alternative Name: Isocitrate Dehydrogenase / IDH

Background: IDH1 belongs to two distinct subclasses. The protein is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. This protein contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.Synonyms: Cytosolic NADP-isocitrate dehydrogenase, ICDH, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase, PICD, cytoplasmic Isocitrate dehydrogenase [NADP]

Gene ID: 3417, 9606

UniProt: O75874