Isocitrate Dehydrogenase antibody

Catalog No. ABIN3197487

This Rabbit Polyclonal antibody specifically detects Isocitrate Dehydrogenase in WB and IF. It exhibits reactivity toward Cabbage, Zea mays, Tomato, Soybean, Red pepper, Potato, Pea, Oryza sativa, Nicotiana benthamiana and Arabidopsis thaliana. It has been mentioned in 3+ publications

Quick Overview for Isocitrate Dehydrogenase antibody (ABIN3197487)

Target: Isocitrate Dehydrogenase (IDH)

Reactivity: Cabbage, Zea mays, Tomato, Soybean, Red pepper, Potato, Pea, Oryza sativa, Nicotiana benthamiana, Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Isocitrate Dehydrogenase antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-Isocitrate Dehydrogenase Antibody

Purpose: Anti-IDH | Isocitrate dehydrogenase (Cellular [compartment marker] of mitochondrial matrix)

Cross-Reactivity (Details): Arabidopsis thaliana, Brassica oleracea, Capsicum annuum, Glycine max, Lycopersicum chilense, Nicotiana benthamiana, Oryza sativa, Solanum lycopersicum, Pisum sativum, Solanum sogarandium, Solanum tuberosum, Zea mays
Not reactive in: Chlamydomonas reinhardtii

Predicted Reactivity: Brachypodium distachyon,Brassica napus,Capsella rubella,Citrus sinensis,Hordeum vulgare,Malus x domestica,Medicago truncatula,Nicotiana tabacum,Phaseolus vulgaris,Theobroma cacao,Triticum aestivum,Vitis vinifera,Zea mays

Purification: Immunogen affinity purified serum

Immunogen: KLH-conjugated peptide 1 and peptide 2 conserved in all higher plants mitochondrial

Application Details

Application Notes: 1:400 (IF), 1 : 5 000 (WB)

Comment:

cellular [compartment marker] of mitochondrial matrix

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References for anti-Isocitrate Dehydrogenase antibody (ABIN3197487)

Rurek, Woyda-Ploszczyca, Jarmuszkiewicz et al.: "Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." in: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, (2015) (PubMed).

Lee, Lee, Yoo, Duncan, Oh, Lee, Lee, Whelan, Hwang: "Mitochondrial targeting of the Arabidopsis F1-ATPase γ-subunit via multiple compensatory and synergistic presequence motifs." in: The Plant cell, Vol. 24, Issue 12, pp. 5037-57, (2013) (PubMed).

Szal, Jastrz?bska, Kulka, Lea: "Influence of mitochondrial genome rearrangement on cucumber leaf carbon and nitrogen metabolism." in: Planta, Vol. 232, Issue 6, pp. 1371-82, (2010) (PubMed).

Target Details for Isocitrate Dehydrogenase

Target: Isocitrate Dehydrogenase (IDH)

Background:

Background: Plant NADH dependent isocitrate dehydrogenase enzyme is located in mitochondrial matrix. This enzyme is classified as an oxidoreductase and its function is to catalyze a reaction in the citric acid cycle, specifically the sequential dehydrogenation and decarboxylation of isocitrate to form a-ketoglutarate. It removes hydrogens from its substrate, isocitrate. In addition to this process, it functions as a decarboxylase, removing a CO2 from the six-carbon substrate to form a five-carbon product mentioned above as a-ketoglutarate. There are two forms of this enzyme NADP+ and NAD+ dependent.

Additional information: Peptide used to elicit this antibody is not conserved in NADPH dependent anzymes, partially conserved across eukaryotic Idh subunits, Some conservation across bacterial which contain the NAD-dependent form of Idh (as opposed to the NADP-dependent form)

Molecular Weight: 39 | 45 kDa (Arabidopsis thaliana)

Gene ID: 816218

UniProt: P93032