MAPK15 antibody (N-Term)

Catalog No. ABIN359709

This anti-MAPK15 antibody is a Rabbit Polyclonal antibody detecting MAPK15 in WB, IHC (p) and EIA. Suitable for Human.

Quick Overview for MAPK15 antibody (N-Term) (ABIN359709)

Target: MAPK15 (Mitogen-Activated Protein Kinase 15 (MAPK15))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAPK15 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-MAPK15 Antibody

Binding Specificity: N-Term

Specificity: This antibody reacts to MAPK15 (ERK8).

Purification: Protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human ERK8.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Immunohistochemistry: 1/10 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for MAPK15

Target: MAPK15 (Mitogen-Activated Protein Kinase 15 (MAPK15))

Alternative Name: MAPK15

Background: The ERKs are a subfamily of the MAPKs that have been implicated in cell growth and differentiation. Extracellular signal-regulated kinase 8 (Erk8) is a large MAP kinase whose activity is controlled by serum and the c-Src non-receptor tyrosine kinase. ERK8 down-regulates transactivation of the glucocorticoid receptor through Hic-5 and can negatively regulate transcriptional co-activation of androgen receptor and GRalpha by Hic-5 in a kinase-independent manner, suggesting a broader role for ERK8 in the regulation of nuclear receptors beyond estrogen receptor alpha. Erk8 is a novel effector of RET/PTC3 and, therefore, RET biological functions.Synonyms: ERK8, Extracellular signal-regulated kinase 8, Mitogen-activated protein kinase 15

Gene ID: 225689, 9606

UniProt: Q8TD08