IARS2 antibody (Middle Region)

Catalog No. ABIN360191

The Rabbit Polyclonal anti-IARS2 antibody is suitable to detect IARS2 in samples from Human. It has been validated for WB, IHC (p) and EIA.

Quick Overview for IARS2 antibody (Middle Region) (ABIN360191)

Target: IARS2 (Isoleucyl-tRNA Synthetase 2, Mitochondrial (IARS2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IARS2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-IARS2 Antibody

Binding Specificity: Middle Region

Specificity: This antibody reacts to IARS2.

Purification: Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human IARS2.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Immunohistochemistry: 1/10 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for IARS2

Target: IARS2 (Isoleucyl-tRNA Synthetase 2, Mitochondrial (IARS2))

Alternative Name: Isoleucyl-tRNA Synthetase / IARS2

Background: IARS2 belongs to the class-I aminoacyl-tRNA synthetase family. Members of class I have two highly conserved sequence motifs. They aminoacylate at the 2'-OH of an adenosine nucleotide, and are usually monomeric or dimeric (one or two subunits, respectively). Both classes of aminoacyl-tRNA synthetases are multidomain proteins. The catalytic domains of all the aaRSs of a given class are found to be homologous to one another, while class I and class II aaRSs are unrelated to one another. The class I aaRSs have the ubiquitous Rossmann fold and have the antiparallel beta-strands architecture while the class II aaRSs have a unique fold made up of antiparallel beta-strands.Synonyms: IleRS, mitochondrial Isoleucine-tRNA ligase

Gene ID: 55699, 9606

UniProt: Q9NSE4