PIK3R1 antibody (Tyr580)

Catalog No. ABIN360457

This Rabbit Polyclonal antibody specifically detects PIK3R1 in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for PIK3R1 antibody (Tyr580) (ABIN360457)

Target: PIK3R1 (PI3K p85a) (Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIK3R1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PIK3R1 Antibody

Binding Specificity: Tyr580

Specificity: This antibody reacts to PIK3R1.

Purification: Protein A column, followed by peptide affinity purification

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding Y580 of human PIK3R1.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Immunohistochemistry: 1/10 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for PIK3R1

Target: PIK3R1 (PI3K p85a) (Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a))

Alternative Name: PIK3R1 / GRB1

Background: Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance.Synonyms: PI3-kinase subunit p85-alpha, PI3K regulatory subunit alpha, Phosphatidylinositol 3-kinase regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha

Gene ID: 5295, 9606

UniProt: P27986

Pathways: TCR Signaling, Response to Growth Hormone Stimulus, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Hepatitis C, Protein targeting to Nucleus, VEGF Signaling, BCR Signaling, Warburg Effect