CIB2 antibody (N-Term)

Catalog No. ABIN360675

Product Details anti-CIB2 Antibody

Target: CIB2 (Calcium And Integrin Binding Family Member 2 (CIB2))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CIB2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts to KIP2.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human KIP2.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for CIB2

Target: CIB2 (Calcium And Integrin Binding Family Member 2 (CIB2))

Alternative Name: CIB2 (CIB2 Products)

Synonyms: DFNB48 antibody, KIP2 antibody, USH1J antibody, 2810434I23Rik antibody, AI449053 antibody, calcium and integrin binding family member 2 antibody, CIB2 antibody, Cib2 antibody

Background: DNA-dependent protein kinases (DNA-PK) play a role in the repair of double-strand DNA breaks and in the process of V(D)J recombination during lymphoid development. By EST database searching for sequences homologous to the DNA-PK gene KIP/CIB and 5-prime RACE, Seki, N et al. isolated a full-length cDNA, which they designated as KIP2, from a human fetal brain cDNA library. KIP2 encodes a deduced 187-amino acid protein with a predicted molecular mass of 22 kD. The KIP2 protein shares 46 % , 39 %, and 30 % sequence identity with the calcium-binding proteins KIP/CIB, calcineurin B and calmodulin respectively. KIP2 contains two EF-hand motifs and a helix-loop-helix motif involved in coordinating the calcium ion, indicating that KIP2 may also bind calcium. KIP2 is ubiquitously expressed in various human tissues.Synonyms: CIB2, Calcium and integrin-binding family member 2, KIP 2, KIP2, Kinase-interacting protein 2

Gene ID: 10518, 9606

UniProt: O75838