Lamin B2 antibody

Catalog No. ABIN360851

The Mouse Monoclonal anti-Lamin B2 antibody is suitable to detect Lamin B2 in samples from Human, Mouse, Zebrafish (Danio rerio), Xenopus laevis, Hamster and Pig. It has been validated for WB, FACS and IHC (fro).

Quick Overview for Lamin B2 antibody (ABIN360851)

Target: Lamin B2 (LMNB2)

Reactivity: Human, Mouse, Zebrafish (Danio rerio), Xenopus laevis, Hamster, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Lamin B2 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: LN43

Product Details anti-Lamin B2 Antibody

Specificity: Reacts with an epitope located in the C-terminal part of Lamin B2.

Purification: Purified

Immunogen: Detergent insoluble fraction of potoroo cell line PtK1.

Isotype: IgG1

Application Details

Application Notes: Suitable for Immunocytochemistry on Frozen Sections, Immunoblotting and FlowCytometry. Recommended Dilutions: 1/100-1/200 for Flow cytometry and for Immunohistochemistry with avidin-biotinylatedhorseradish peroxidase complex (ABC) as detection reagent.1/100-1/1000 for Immunoblotting applications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS with 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized at 2 - 8 °C and reconstituted at -20 °C.

Target Details for Lamin B2

Target: Lamin B2 (LMNB2)

Alternative Name: Lamin-B2 (LMNB2)

Background: An important part of the cell nucleus is formed by nuclear lamina. Nuclear lamins form a network of filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. The nuclear lamins comprise a unique subclass of the intermediate filament protein family. They share a molecular domain organisation with the other intermediate filament proteins in that they are fibrous molecules that have an aminoterminal globular head, a central rod of a-helices and a carboxyterminal globular domain. Many biochemical and molecular features of lamins have been studied, but their functions remain still largely undetermined. One of the functions ascribed to the lamina is the maintenance of the structural integrity of the nucleus. Besides interactions with the nuclear membrane and other intermediate filaments, lamins interact with the nuclear chromatin. Eukaryotic chromatin is organised into loops, which are attached to the nuclear matrix. This organisation is thought to contribute to compaction of the chromatin and regulation of gene expression. Lamins, as part of the nuclear matrix, may be involved in these processes since chromatin binding sites have been detected in both A- and B-type lamins.Synonyms: LMN2, LMNB, LMNB-2, LMNB2, Lamin-B2, Nuclear Envelope Marker

Gene ID: 84823

NCBI Accession: NP_116126

UniProt: Q03252

Pathways: Apoptosis, Caspase Cascade in Apoptosis