desert Hedgehog antibody (N-Term)

Catalog No. ABIN360880

This Rat Monoclonal antibody specifically detects desert Hedgehog in WB. It exhibits reactivity toward Mouse.

Quick Overview for desert Hedgehog antibody (N-Term) (ABIN360880)

Target: desert Hedgehog (DHH) (Desert Hedgehog (DHH))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This desert Hedgehog antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 6A12

Product Details anti-desert Hedgehog Antibody

Binding Specificity: N-Term

Specificity: This antibody detects Mouse DHH N-terminal polypeptide, but not C-terminal one in Western blotting.

Purification: Protein A/G affinity chromatography

Immunogen: Recombinant dhh N-terminal fragment

Isotype: IgG2

Application Details

Application Notes: Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 200 μL sterile PBS and the final concentration is 500 μg/mL.

Buffer: 0.2 μm filtered PBS solution, None

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for desert Hedgehog

Target: desert Hedgehog (DHH) (Desert Hedgehog (DHH))

Alternative Name: Desert Hedgehog / DHH

Target Type: Species

Background: Desert Hedgehog (Dhh) belongs to the highly conserved Hedgehog family of proteins which are involved in multiple developmental processes. Hedgehogs are synthesized as 45 kDa precursors that are cleaved autocatalytically. The 19 kDa Nterminal fragment remains membrane associated due to its cholesterol and palmitate modifications. Binding of this fragment to Patched receptors results in the loss of Patched repression of Smoothened signaling. Dhh binds both Patched and Patched 2 as well as Hedgehog interacting protein (Hip). Within the N-terminal peptide, mouse Dhh shares 97 % and 100 % amino acid (aa) sequence identity with human and rat Dhh, respectively. It shares 74 % aa seqeuence identity with mouse Indian (Ihh) and Sonic hedgehog (Shh). Dhh is produced by Sertoli cells and is required for testis development and spermatogenesis. It induces steroidogenic factor 1 which is instrumental in promoting Leydig cell differentiation. It also promotes the deposition of basal lamina surrounding seminiferous tubules. In humans, mutations of Dhh are associated with pure gonadal dysgenesis. Dhh is expressed in the female by ovarian granulosa cells and the corpus luteum. Its upregulation in human ovarian cancer correlates positively with proliferative index and negatively with prognosis. Dhh is also expressed by Schwann cells and is upregulated following nerve injury. It induces the expression of Patched and Hip in nerve fibroblasts and promotes the formation of the connective tissue sheath surrounding peripheral nerves.Synonyms: HHG-3

Gene ID: 13363

NCBI Accession: NP_031883

UniProt: Q61488

Pathways: Hedgehog Signaling