LGALS1/Galectin 1 antibody

Catalog No. ABIN360886

This Rat Monoclonal antibody specifically detects LGALS1/Galectin 1 in WB and IHC (fro). It exhibits reactivity toward Mouse.

Quick Overview for LGALS1/Galectin 1 antibody (ABIN360886)

Target: LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This LGALS1/Galectin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 9J26

Product Details anti-LGALS1/Galectin 1 Antibody

Specificity: This antibody detects Mouse Galectin-1. Other species not tested.

Purification: Protein A/G Affinity Chromatography

Sterility: 0.2 μm filtered

Immunogen: Purified Recombinant Mouse Galectin-1 Remarks: Mouse myeloma fused with spleen cells from a rat

Isotype: IgG2

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry on frozen sections: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 200 μL sterile PBS and the final concentration is 500 μg/mL.

Buffer: 0.2 μm filtered solution in PBS without preservatives or stabilizers

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for LGALS1/Galectin 1

Target: LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

Alternative Name: Galectin-1

Background: The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin 1 may act as an autocrine negative growth factor that regulates cell proliferation. It also may regulate cell apoptosis and cell differentiation. It binds beta-galactoside, CD45, CD3 and CD4. Galectins contain either one or two carbohydrate recognition domains (CRR) which mediate recognition of N-acetyl-lactosamine-containing glycoproteins. Some galectins exist in multiple isoforms due to alternative splicing. Individual galectins differ in their tissue distribution and in their carbohydrate-binding specificities. Galectin 1 inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. It is found in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney, placenta and hematopoietic cells.Synonyms: 14 kDa lectin, Beta-galactoside-binding lectin L-14-I, Galaptin, Galectin1, HBL, HPL, LGALS1, Lactose-binding lectin 1, Lectin galactoside-binding soluble 1, MAPK-activating protein PM12, S-Lac lectin 1

Gene ID: 16852

NCBI Accession: NP_032521

UniProt: P16045

Pathways: Carbohydrate Homeostasis