Ly6g antibody

Catalog No. ABIN360930

The Rat Monoclonal anti-Ly6g antibody (Clone 6D17) (ABIN360930) specifically detects Ly6g in FACS and IHC (p). The antibody is reactive with Mouse samples.

Quick Overview for Ly6g antibody (ABIN360930)

Target: Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Ly6g antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 6D17

Product Details anti-Ly6g Antibody

Cross-Reactivity (Details): Species reactivity (tested):Mouse.

Purification: Affinity Chromatography on Protein A/G.

Immunogen: Cell lysates of Mouse Neutrophils. Remarks: Hybridoma: Mouse myeloma fused with spleen cells from a Rat.

Isotype: IgG2

Application Details

Application Notes: FACS analysis: 1/500-1/2000. Immunohistochemistry on Paraffin Sections: 1/100-1/400.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 250 μL sterile PBS (final concentration = 400 μg/mL).

Buffer: PBS without additives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Ly6g

Target: Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))

Alternative Name: Ly6G / GR1 Neutrophil Marker

Background: Gr-1 is a 21-25 kD protein also known as Ly-6G/Ly-6C. This myeloid differentiation antigen is a glycosylphosphatidylinositol (GPI)-linked protein expressed on granulocytes and macrophages. In bone marrow, the expression levels of Gr-1 directly correlate with granulocyte differentiation and maturation. Gr-1 is also transiently expressed on bone marrow cells in the monocyte lineage. Gr-1+ cells have recently been implicated in the development of antitumor responses.Synonyms: Gr-1 Granulocyte marker

Gene ID: 10090

UniProt: P35461