RAGE antibody (Extracellular Domain)

Catalog No. ABIN360934

This Rat Monoclonal antibody specifically detects RAGE in WB, IF and IHC (fro). It exhibits reactivity toward Mouseand has been mentioned in 1 publication.

Quick Overview for RAGE antibody (Extracellular Domain) (ABIN360934)

Target: RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This RAGE antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 2B65

Product Details anti-RAGE Antibody

Binding Specificity: Extracellular Domain

Specificity: This antibody detects RAGE.

Purification: Protein A/G Affinity Chromatography

Immunogen: Mouse Recombinant RAGE extracellular domain. Remarks: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from an immunized rat).

Isotype: IgG2

Application Details

Application Notes: Western Blot: 1/500-1/1000. Immunohistochemistry on Frozen Sections: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 200 μL sterile PBS and the final concentration is 500 μg/mL.

Concentration: 0,5 mg/mL

Buffer: PBS without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-RAGE antibody (ABIN360934)

Aue, Englert, Harrer, Schwiering, Gaab, König, Adams, Schmidtko, Friebe, Groneberg: "NO-sensitive guanylyl cyclase discriminates pericyte-derived interstitial from intra-alveolar myofibroblasts in murine pulmonary fibrosis." in: Respiratory research, Vol. 24, Issue 1, pp. 167, (2023) (PubMed).

Target Details for RAGE

Target: RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

Alternative Name: RAGE / AGER

Background: The Receptor for Advanced Glycation Endproducts (RAGE) is a member of the Immunoglobin superfamily of cell surface markers. This protein is able to interact with many molecules including: advanced glycation endproducts (AGE), amphoterin, and ligands. Research has shown that the accumulation of RAGE ligands in the biological system (endothelium mononuclear phagocytes, neurons and smooth muscle cells) can lead to destructive tissue diseases such as diabetic retinopathy, amyloidoses, tumors and inflammation disorders. RAGE is expressed by endothelium mononuclear phagocytes, smooth muscle and neurons.Synonyms: Advanced glycosylation end product-specific receptor, Receptor for advanced glycosylation end products

Gene ID: 10090

UniProt: Q62151

Pathways: Carbohydrate Homeostasis, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, S100 Proteins