RAGE antibody (AA 151-250)

Catalog No. ABIN725900

There are 5+ publications for this product available. The Rabbit Polyclonal anti-RAGE antibody is suitable to detect RAGE in samples from Rat. It has been validated for WB, IP, IHC (p), IF (cc), IF (p) and IHC (fro).

Quick Overview for RAGE antibody (AA 151-250) (ABIN725900)

Target: RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAGE antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-RAGE Antibody

Binding Specificity: AA 151-250

Purpose: RAGE Polyclonal Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from rat AGER

Isotype: IgG

Application Details

Application Notes: WB(1:300-5000),

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-RAGE antibody (ABIN725900)

Fu, Shi, Song, Liu, Wang, Wang, Song, Song: "From the Cover: Tetrachlorobenzoquinone Exerts Neurological Proinflammatory Activity by Promoting HMGB1 Release, Which Induces TLR4 Clustering within the Lipid Raft." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 153, Issue 2, pp. 303-15, (2016) (PubMed).

Sun, Liu, Xue, Wang: "Effects of insulin combined with idebenone on blood-brain barrier permeability in diabetic rats." in: Journal of neuroscience research, Vol. 93, Issue 4, pp. 666-77, (2015) (PubMed).

Zhu, Yao, Zhang, Dong, Yu, Sheng: "Anti-RAGE antibody ameliorates severe thermal injury in rats through regulating cellular immune function." in: Acta pharmacologica Sinica, Vol. 35, Issue 9, pp. 1167-76, (2014) (PubMed).

Yu, Wu, Cai, Xiang, Zha, Fan, Guo, Ming, Liu: "Curcumin alleviates diabetic cardiomyopathy in experimental diabetic rats." in: PLoS ONE, Vol. 7, Issue 12, pp. e52013, (2012) (PubMed).

Huang, Yao, Zhang, Dong, Yu, Sheng: "The effect of high-mobility group box 1 protein on activity of regulatory T cells after thermal injury in rats." in: Shock (Augusta, Ga.), Vol. 31, Issue 3, pp. 322-9, (2009) (PubMed).

Target Details for RAGE

Target: RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

Alternative Name: RAGE

Background:

Synonyms: RAGE, Advanced glycosylation end product-specific receptor, Receptor for advanced glycosylation end products, Ager

Background: Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S1A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Can also bind oligonucleotides (By similarity). Interaction with S1B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling.

Gene ID: 81722

UniProt: Q63495

Pathways: Carbohydrate Homeostasis, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, S100 Proteins