FLT1 antibody (C-Term)

Catalog No. ABIN360964

This Rabbit Polyclonal antibody specifically detects FLT1 in WB and IF. It exhibits reactivity toward Human.

Quick Overview for FLT1 antibody (C-Term) (ABIN360964)

Target: FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FLT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-FLT1 Antibody

Binding Specificity: C-Term

Sequence: CNKKAVFSRI SKFKSTRNDS TTQSNVKH

Specificity: This antibody recognizes the recombinant soluble VEGFR-1/FLT-1 (native) but not the recombinant soluble VEGFR-1/FLT-1 (D1-5) both produced in Insects cells. Because of the different glycosylation of insect cells compared to mammalian cells the MW of sFlt-1 (native) is about 96 kDa instead of about 110 kDa of the naturally occurring sFlt-1. The antibody did not cross react with recombinant Human soluble KDR (D1-7).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Protein A Chromatography.

Immunogen: Peptide of the C-terminal end of native Human soluble VEGFR-1/Flt-1

Isotype: IgG

Application Details

Application Notes: Western Blot: This VEGFR1 antibody can be used at 0.5-2.0 μg/mL with the appropriatesecondary reagents to detect native Human sVEGFR-1/Flt-1. Immunofluorescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of > 0.5 mg/mL

Buffer: PBS, pH 7.2 without preservatives or stabilizers.

Preservative: Without preservative

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Target Details for FLT1

Target: FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))

Alternative Name: VEGFR-1 / Flt-1

Background: Three cell membrane receptor tyrosine kinases, Flt (also designated VEGF-R1), Flk-1 (also designated VEGF-R2) and Flt-4, putatively involved in the growth of endothelial cells, are characterized by the presence of seven immunoglobulinlike sequences in their extracellular domain. These receptors exhibit high degrees of sequence relatedness to each other as well as lesser degrees of relatedness to the class III receptors, including CSF-1/Fms, PDGR, SLFR/Kit and Flt-3/Flk-2. Two members of this receptor class, Flt-1 and Flk-1, have been shown to represent high affinity receptors for vascular endothelial growth factors (VEGFs). On the basis of structural similarity to Flt and Flk-1, it has been speculated that Flt-4 might represent a third receptor for either VEGF or a VEGF-related ligand.Synonyms: FLT, FLT1, FRT, Fms-like tyrosine kinase 1, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, VEGF Receptor 1, VEGFR1, Vascular endothelial growth factor receptor 1, Vascular permeability factor receptor

Gene ID: 2321

NCBI Accession: NP_001153392

UniProt: P17948

Pathways: RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals