DLL1 antibody (Internal Region)

Catalog No. ABIN361190

The Goat Polyclonal anti-DLL1 antibody has been validated for WB, ELISA, FACS and IF. It is suitable to detect DLL1 in samples from Human and Rat.

Quick Overview for DLL1 antibody (Internal Region) (ABIN361190)

Target: DLL1 (delta-Like 1 (DLL1))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This DLL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Verified

Product Details anti-DLL1 Antibody

Binding Specificity: Internal Region

Purpose: DLL1

Sequence: ATQRHLTVGE EWSQD

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-ATQRHLTVGEEWSQD, from the internal region of the protein sequence according to NP_005609.3

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 75 kDa band observed in Mouse Heart and Rat Pancreas lysates (calculated MW of 78.5 kDa according to Mouse NP_031891.2 and 77.4 kDa according to Rat NP_114452.1). An additional band of unknown identity was also consistently observed at

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ membranes of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for DLL1

Target: DLL1 (delta-Like 1 (DLL1))

Alternative Name: DLL1

Background: DLL1, delta-like 1 (Drosophila), DELTA1, DL1, Delta, OTTHUMP00000017690, delta (Drosophila)-like 1, delta-like 1, delta-like 1 protein

Gene ID: 28514, 13388, 84010

NCBI Accession: NP_005609

Pathways: Notch Signaling, Stem Cell Maintenance