PON1 antibody (Internal Region)

Catalog No. ABIN361191

This anti-PON1 antibody is a Goat Polyclonal antibody detecting PON1 in WB and ELISA. Suitable for Human.

Quick Overview for PON1 antibody (Internal Region) (ABIN361191)

Target: PON1 (Paraoxonase 1 (PON1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PON1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-PON1 Antibody

Binding Specificity: Internal Region

Purpose: PON1 / paraoxonase 1

Sequence: EEPKVTQVYA EN

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EEPKVTQVYAEN, from the internal region of the protein sequence according to NP_000437.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx. 37 kDa band observed in Human Liver lysates (calculated MW of 39.7 kDa according to NP_000437.3). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:2000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PON1

Target: PON1 (Paraoxonase 1 (PON1))

Alternative Name: PON1

Background: PON1, paraoxonase 1, ESA, PON, esterase A

Gene ID: 5444

NCBI Accession: NP_000437