Actin antibody

Catalog No. ABIN361349

The Mouse Monoclonal anti-Actin antibody has been validated for WB, IHC, ELISA and ICC. It is suitable to detect Actin in samples from Chicken. There are 5+ publications available.

Quick Overview for Actin antibody (ABIN361349)

Target: Actin

Reactivity: Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)

Clone: C4

Product Details anti-Actin Antibody

Specificity: Specific for endogenous levels of the ~42 kDa actin protein. Reacts with all actin isoforms.

Cross-Reactivity: Various Species

Characteristics: Our Anti-Actin Antibody primary antibody is mouse monoclonal. It detects all known species Actin and is mouse ascites fluid. It is great for use in ELISA, WB, IHC, ICC.

Purification: Unpurified ascites fluid.

Immunogen: Preparation of chicken gizzard actin

Isotype: IgG1

Application Details

Application Notes:

Dilution Range: ICC: 1:200-1:500

Dilution Range: WB Brain: 1:5000

Dilution Range: WB: 1:5000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Ascites fluid + 0.01 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Expiry Date: 12 months

References for anti-Actin antibody (ABIN361349)

Wheaton, Aten, Queiroz, Sullivan, Oberdick, Hoyt, Obrietan: "Circadian expression and functional characterization of PEA-15 within the mouse suprachiasmatic nucleus." in: The European journal of neuroscience, Vol. 47, Issue 7, pp. 845-857, (2019) (PubMed).

Choi, Horning, Aten, Karelina, Alzate-Correa, Arthur, Hoyt, Obrietan: "Mitogen- and Stress-Activated Protein Kinase 1 Regulates Status Epilepticus-Evoked Cell Death in the Hippocampus." in: ASN neuro, Vol. 9, Issue 5, pp. 1759091417726607, (2018) (PubMed).

Barcus, OLeary, Brockman, Rugowski, Liu, Garcia, Yu, Keely, Eliceiri, Schuler: "Elevated collagen-I augments tumor progressive signals, intravasation and metastasis of prolactin-induced estrogen receptor alpha positive mammary tumor cells." in: Breast cancer research : BCR, Vol. 19, Issue 1, pp. 9, (2017) (PubMed).

Ramena, Yin, Yu, Walia, Elble: "CLCA2 Interactor EVA1 Is Required for Mammary Epithelial Cell Differentiation." in: PLoS ONE, Vol. 11, Issue 3, pp. e0147489, (2016) (PubMed).

Barcus, Holt, Keely, Eliceiri, Schuler: "Dense collagen-I matrices enhance pro-tumorigenic estrogen-prolactin crosstalk in MCF-7 and T47D breast cancer cells." in: PLoS ONE, Vol. 10, Issue 1, pp. e0116891, (2016) (PubMed).

Target Details for Actin

Target: Actin

Background:

Synonyms: a actin antibody, ACTA antibody, ACTA1 antibody, Actin alpha skeletal muscle antibody, Actin antibody, actin alpha 1 skeletal muscle 1 antibody, actin alpha 1 skeletal muscle antibody, Actin alpha skeletal muscle antibody, actina antibody, actine antibody, ACTS_HUMAN antibody, aktin antibody, Alpha Actin 1 antibody, Alpha skeletal muscle Actin antibody, alpha skeletal muscle antibody, alpha-actin antibody, Alpha-actin-1 antibody, ASMA antibody, CFTD antibody, CFTD1 antibody, CFTDM antibody, MPFD antibody, NEM1 antibody, NEM2 antibody, NEM3 antibody, nemaline myopathy type 3 antibody

Description Logic: Our Anti-Actin Antibody primary antibody is mouse monoclonal. It detects all known species Actin and is mouse ascites fluid. It is great for use in ELISA, WB, IHC, ICC.

Gene Name Alternatives: ACTA1

Molecular Weight: 42 kDa

Gene ID: 421534

UniProt: P68139