NEFL antibody

Catalog No. ABIN361353

This anti-NEFL antibody is a Chicken Polyclonal antibody detecting NEFL in WB and IF. Suitable for Cow.

Quick Overview for NEFL antibody (ABIN361353)

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Reactivity: Cow

Host: Chicken

Clonality: Polyclonal

Conjugate: This NEFL antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-NEFL Antibody

Specificity: Specific for the ~68k Neurofilament L protein in Western blots and works well on frozen sections, cells in tissue culture and on mildly formalin fixed histological sections.

Cross-Reactivity: Chicken, Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)

Purification: Total IgY fraction

Immunogen: Preparation of bovine spinal cord NF-L

Application Details

Application Notes: Recommended Dilution: WB: 1:10,000 IF: 1:5,000 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: total IgY fraction in PBS + 10 mM Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for NEFL

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Alternative Name: NEFL

Background: Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. Mutations in the protein coding region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease (2). Anti-Neurofilament L Western blot of rat cortex lysate showing specific immunolableing of the ~ 68k NF-L protein.

Molecular Weight: '68 kDa

Gene ID: 4747

UniProt: P02548