NEFM antibody (C-Term)
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- Target See all NEFM Antibodies
- NEFM (Neurofilament Triplet M Protein (NEFM))
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Binding Specificity
- C-Term
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Reactivity
- Rat
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Host
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Chicken
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Clonality
- Polyclonal
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Conjugate
- This NEFM antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF)
- Specificity
- Specific for the ~145k Neurofilament M protein.
- Cross-Reactivity
- Chicken, Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
- Purification
- Total IgY fraction
- Immunogen
- Preparation containing the extreme C-terminus expressed in and purified from E. Coli
- Top Product
- Discover our top product NEFM Primary Antibody
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- Application Notes
- Recommended Dilution: WB: 1:5,000 IF: 1:1000 Quality Control: Western blots performed on each lot.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- total IgY fraction in PBS + 10 mM Sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
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Functional characterization of extrinsic tongue muscles in the Pink1-/- rat model of Parkinson disease." in: PloS one, Vol. 15, Issue 10, pp. e0240366, (2020) (PubMed).
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Functional characterization of extrinsic tongue muscles in the Pink1-/- rat model of Parkinson disease." in: PloS one, Vol. 15, Issue 10, pp. e0240366, (2020) (PubMed).
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- Target
- NEFM (Neurofilament Triplet M Protein (NEFM))
- Alternative Name
- NEFM (NEFM Products)
- Background
- Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease (2). Anti-Neurofilament M Western blot of rat cortex lysate showing specific immunolableing of the ~ 145k NF-M protein.
- Molecular Weight
- '145 kDa
- Gene ID
- 24588
- UniProt
- P12839
- Pathways
- Brown Fat Cell Differentiation
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