Nuclei antibody

Catalog No. ABIN361360

This Mouse Monoclonal antibody specifically detects not_set in IHC, ICC and ELISA. It exhibits reactivity toward Human and has been mentioned in 4+ publications.

Quick Overview for Nuclei antibody (ABIN361360)

Target: Nuclei

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Immunocytochemistry (ICC), ELISA

Clone: 235-1

Product Details

Specificity: Specific for endogenous levels of human nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. With immunoprecipitation, the antibody reacts with a ~80 kDa and ~70 kDa band. The antibody works on acetone fixed and paraformaldehyde fixed cells. It is suggested that you permeabilize the cells with 0.1-0.4 % Triton X100. The antibody also works for immunohistochemistry on paraformaldehyde fixed cryostat sections.

Cross-Reactivity: Human

Characteristics: Our Anti-Nuclei Antibody primary antibody is mouse monoclonal. It detects human Nuclei and is concentrated tissue culture supernatant. It is great for use in ELISA, IHC, ICC.

Purification: Concentrated tissue ulture supernatant.

Immunogen: Human cell homogenate.

Isotype: IgG1

Application Details

Application Notes: 1:100

Comment:

Normally ABIN361360 should be fix with 10% buffered formalin overnight at room temperature instead of 4% paraformaldehyde. 1.Use 10% buffered formalin overnight instead of 4% paraformaldehyde to fix the tissues 2.Quench the endogenous peroxidase activity with 3% H2O2 instead of 0.5% H2O2 3.Block non-specific binding sites with 3% horse serum instead of goat serum.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Concentrated tissue culture supernatent

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Expiry Date: 12 months

References

Subramanian, Sidhom, Emani, Vernon, Sahakian, Zhou, Kost-Alimova, Slyper, Waldman, Dionne, Nguyen, Weins, Marshall, Rosenblatt-Rosen, Regev, Greka: "Single cell census of human kidney organoids shows reproducibility and diminished off-target cells after transplantation." in: Nature communications, Vol. 10, Issue 1, pp. 5462, (2019) (PubMed).

van den Berg, Ritsma, Avramut, Wiersma, van den Berg, Leuning, Lievers, Koning, Vanslambrouck, Koster, Howden, Takasato, Little, Rabelink: "Renal Subcapsular Transplantation of PSC-Derived Kidney Organoids Induces Neo-vasculogenesis and Significant Glomerular and Tubular Maturation In Vivo." in: Stem cell reports, Vol. 10, Issue 3, pp. 751-765, (2018) (PubMed).

Balseanu, Buga, Catalin, Wagner, Boltze, Zagrean, Reymann, Schaebitz, Popa-Wagner: "Multimodal Approaches for Regenerative Stroke Therapies: Combination of Granulocyte Colony-Stimulating Factor with Bone Marrow Mesenchymal Stem Cells is Not Superior to G-CSF Alone." in: Frontiers in aging neuroscience, Vol. 6, pp. 130, (2014) (PubMed).

Kim, Godarova, Seedle, Kim, Ince, Wells, Driscoll, Godar: "Rb suppresses collective invasion, circulation and metastasis of breast cancer cells in CD44-dependent manner." in: PLoS ONE, Vol. 8, Issue 12, pp. e80590, (2014) (PubMed).

Target Details

Target: Nuclei

Background:

Synonyms: HNA antibody, Human Nuclear Antigen antibody, HuNu antibody

Description Logic: Our Anti-Nuclei Antibody primary antibody is mouse monoclonal. It detects human Nuclei and is concentrated tissue culture supernatant. It is great for use in ELISA, IHC, ICC.

Gene Name Alternatives: Marker for human cells in xenographic research