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- Target
- Nuclei
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- Immunohistochemistry (IHC), Fluorescence Microscopy (FM)
- Purification
- Anti-Nuclei antibody is directed against nuclei proteins. This monoclonal antibody is supplied as a concentrated cell culture supernatant. This antibody stains nuclei of all human cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. By immunoprecipitation, the antibody reacts with an 80 kDa and 70 kDa band. Anti-Nuclei antibody works on acetone fixed and paraformaldehyde fixed cells. It is suggested that you permeabilize the cells with 0.1-0.4% Triton X100. This antibody also works for immunohistochemistry on paraformaldehyde fixed cryostat sections. Anti-Nuclei Antibody is ideal for investigators involved in Cell Cycle, Cancer, and Neuroscience Research.
- Immunogen
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Immunogen: Anti-Nuclei Antibody (Monoclonal) was produced by repeated immunizations with human cell homogenate.
Immunogen Type: Native Protein
- Clone
- 235-1
- Isotype
- IgG
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anti-Nuclei antibody
Reactivity: Human IF, IHC Host: Mouse Monoclonal 235-1 unconjugated
anti-Nuclei antibody (PE)Reactivity: Human IF, FACS Host: Mouse Monoclonal 235-1 PE
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- Application Notes
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Immunohistochemistry Dilution: 1:100
Application Note: Anti-Nuclei (Mouse) antibody is suitable for use in IHC and IF. Specific conditions for reactivity should be optimized by the end user. This antibody is directed against human nuclei.
IF Microscopy Dilution: 1:100
- Restrictions
- For Research Use only
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- Format
- Liquid
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
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- Target
- Nuclei
- Background
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Synonyms: mouse anti-Nuclei Antibody, HuNu, marker for human cells in xenographic research
Background: Anti-Nuclei Antibody detects nuclei proteins. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells, with no reactivity to rat or mouse cells.
Gene Name: marker for human cells in xenographic research
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