Nuclei antibody

Catalog No. ABIN2743377

Product Details

Target: Nuclei

Reactivity: Human, Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Human Nuclear Marker 235-1

No Cross-Reactivity: Chicken, Mouse (Murine), Rat (Rattus)

Purification: Protein G purified

Immunogen: Nuclei of human myeloid leukemia biopsy cells were used as the immunogen for this Nuclear marker antibody.

Type of Immunogen: Cells

Clone: 235-1

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: Flo (0.5 - 1 μg/10 x10⁶), ICC (0.25 - 0.5 μg/mL), IF (0.5 - 1 μg/mL), IHC, IHC-P (0.5 - 1 μg/mL), IP

Usage: This Nuclear marker antibody is part of a panel of reagents which recognizes subcellular organelles or compartments. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. Clone 235-1 antibody specifically detects an antigen associated with the human nuclei. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. The Nuclear marker antibody can also be used with paraformaldehyde fixed frozen tissue or cell preparations. Immunocytochemistry (Acetone-fixed) : 0.25-0.5 μg/mL for 30 minutes at RT.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 mg/mL BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2°C to 8°C.

Target Details

Target: Nuclei