Specific for the ~55kDa beta III tubulin protein. This clone is similar to the monoclonal antibody Tuj1. It is expected that this antibody will have wide species cross reactivity.
Cross-Reactivity
Rat (Rattus)
Purification
Protein G purified culture supernatant
Immunogen
Synthetic peptide corresponding to amino acid residues specific to beta III tubulin conjugated to KLH
TUBB3
Reactivity: Human
WB, ELISA, IHC, IF, IP
Host: Mouse
Monoclonal
unconjugated
Application Notes
Recommended Dilution: WB: 1:10,000 IHC: 1:1,000 Quality Control: Western blots performed on each lot.
Restrictions
For Research Use only
Format
Liquid
Buffer
100 μL in 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.
Storage
-20 °C
Moldovan, Al-Ebraheem, Lobo, Park, Farquharson, Bock: "Altered transition metal homeostasis in the cuprizone model of demyelination." in: Neurotoxicology, Vol. 48, pp. 1-8, (2015) (PubMed).
Stallings, Puttaparthi, Dowling, Luther, Burns, Davis, Elliott: "TDP-43, an ALS linked protein, regulates fat deposition and glucose homeostasis." in: PLoS ONE, Vol. 8, Issue 8, pp. e71793, (2014) (PubMed).
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Background
Tubulin is the major constituent of microtubules, existing as a heterodimer of the alpha and beta subunits. The beta III isoform of tubulin is found almost exclusively in neuronal processes of adult tissues and is therefore an excellent marker for neurons. Neuron specific, posttranslational modifications within the C-terminal domain of beta III tubulin have been shown to be developmentally regulated suggesting that they may serve to modulate the interaction of tubulin with microtubule associated proteins (Lee et al., 1990). Additionally, beta III tubulin has been found to be highly expressed in cancer cells such as small cell lung cancer, large cell neuroendocrine carcinoma and adenocarcinomas and is correlated with an increasing histological degree of malignancy (Katsetos et al., 2003) Anti-Beta III Tubulin Western blot of rat cortex lysate showing specific immunolabeling of the ~55k beta III tubulin protein.