GAPDHS antibody

Catalog No. ABIN361377

This anti-GAPDHS antibody is a Mouse Monoclonal antibody detecting GAPDHS in WB and IF. Suitable for Pig.

Quick Overview for GAPDHS antibody (ABIN361377)

Target: GAPDHS (Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS))

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAPDHS antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 1D4

Product Details anti-GAPDHS Antibody

Specificity: Specific for the ~38k Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) protein.

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Avian, bovine

Purification: Total IgM fraction--concentrated culture supernatant

Immunogen: Purified porcine GAPDH

Isotype: IgM

Application Details

Application Notes: Recommended Dilution: WB: 1:1,000 IF: 1:100 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: total IgM fraction + 10 mM Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for GAPDHS

Target: GAPDHS (Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS))

Alternative Name: GAPDHS

Background: Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH is a protein expressed in large amounts and which is required at all times for important house keeping functions, levels of GAPDH mRNA are often measured and used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH in comparable studies of levels of protein expression. This antibody can be used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue. Apart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription (1). GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease (2,3). Associations with actin and tubulin have also been reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic (4). Purification: Unpurified, concentrated culture supernatant.

Molecular Weight: '38 kDa

Gene ID: 26330

UniProt: O14556

Pathways: Regulation of Carbohydrate Metabolic Process