NMDAR2A antibody (N-Term)

Catalog No. ABIN361397

The Rabbit Polyclonal anti-NMDAR2A antibody has been validated for WB and IHC. It is suitable to detect NMDAR2A in samples from Rat.

Quick Overview for NMDAR2A antibody (N-Term) (ABIN361397)

Target: NMDAR2A (GRIN2A) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NMDAR2A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NMDAR2A Antibody

Binding Specificity: N-Term

Specificity: Specific for the ~180k NR2A subunit of the NMDA receptor.

Cross-Reactivity: Rat (Rattus)

Predicted Reactivity: bovine, canine, mouse

Purification: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic peptide corresponding to amino acid residues from the N-terminal region of the NR2A subunit conjugated to KLH

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 IHC: 1:500 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Target Details for NMDAR2A

Target: NMDAR2A (GRIN2A) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A))

Alternative Name: GRIN2A

Background: The ion channels activated by glutamate are typically divided into two classes. Glutamate receptors that are activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002, Wenthold et al., 2003, Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989, Alvestad et al., 2003, Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits. Anti-NMDA Receptor, NR2A Subunit Western blot of 10 ug of rat hippocampal lysate showing specific immunolabeling of the ~180k NR2A subunit of the NMDA receptor.

Molecular Weight: '180 kDa

Gene ID: 24409

UniProt: Q00959

Pathways: Synaptic Membrane, Regulation of long-term Neuronal Synaptic Plasticity