CREB1 antibody (pSer133)

Catalog No. ABIN361461

Cited in 1 publication. The Rabbit Polyclonal anti-CREB1 antibody (ABIN361461) specifically detects CREB1 in WB, IHC and ELISA. The antibody is reactive with Rat samples.

Quick Overview for CREB1 antibody (pSer133) (ABIN361461)

Target: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CREB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-CREB1 Antibody

Binding Specificity: pSer133

Specificity: Specific for endogenous levels of the ~45 kDa CREB protein phosphorylated at Ser133. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: bovine, canine, chicken, human, mouse, non-human primate, rat, sheep, Xenopus, zebra fish

Characteristics: Our CREB (Ser133) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects CREB (Ser133) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, IHC.

Purification: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser133 of rat CREB, conjugated to keyhole limpet hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

Dilution Range: WB Brain: 1:1000

Dilution Range: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Expiry Date: 12 months

References for anti-CREB1 antibody (ABIN361461)

Ahmed, Gardiner: "Preserving protein profiles in tissue samples: differing outcomes with and without heat stabilization." in: Journal of neuroscience methods, Vol. 196, Issue 1, pp. 99-106, (2011) (PubMed).

Target Details for CREB1

Target: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Alternative Name: CREB1

Background:

Synonyms: Active transcription factor CREB antibody, cAMP response element binding protein 1 antibody, cAMP response element binding protein antibody, cAMP responsive element binding protein 1 antibody, cAMP-responsive element-binding protein 1 antibody, CREB antibody, CREB-1 antibody, CREB1 antibody, CREB1_HUMAN antibody, Cyclic AMP-responsive element-binding protein 1 antibody, MGC9284 antibody, OTTHUMP00000163864 antibody, OTTHUMP00000163865 antibody, OTTHUMP00000206660 antibody, OTTHUMP00000206662 antibody, , TTHUMP00000206667 antibody, Transactivator protein antibody

Description Logic: Our CREB (Ser133) rabbit polyclonal phosphospecific primary antibody is produced . It detects CREB (Ser133) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, IHC.

Gene Name Alternatives: CREB1

Molecular Weight: 45 kDa

Gene ID: 81646

UniProt: P15337

Pathways: TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation