Glutamate Receptor 1 antibody (pSer831)

Catalog No. ABIN361476

Cited in 9+ publications. This anti-Glutamate Receptor 1 antibody is a Rabbit Polyclonal antibody detecting Glutamate Receptor 1 in WB and ELISA. Suitable for Rat.

Quick Overview for Glutamate Receptor 1 antibody (pSer831) (ABIN361476)

Target: Glutamate Receptor 1 (GLUR1)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glutamate Receptor 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Glutamate Receptor 1 Antibody

Binding Specificity: pSer831

Specificity: Specific for endogenous levels of the ~100 kDa GluR1 protein phosphorylated at Ser831. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with canine, chicken, human and mouse based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Predicted Reactivity: canine, chicken, human

Characteristics: Our GluR1-Subunit (Ser831) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects GluR1-Subunit (Ser831) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Purification: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser831 of rat GluR1 conjugated to keyhole limpet hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

Dilution Range: WB Brain: 1:1000

Dilution Range: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Handling Advice: Avoid repeated freezing and thawing

Storage: -20 °C

Storage Comment: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Expiry Date: 12 months

References for anti-Glutamate Receptor 1 antibody (ABIN361476)

Mao, Wang: "Upregulation of AMPA receptor GluA1 phosphorylation by blocking adenosine A1 receptors in the male rat forebrain." in: Brain and behavior, Vol. 10, Issue 3, pp. e01543, (2020) (PubMed).

Xue, Chen, He, Jin, Mao, Wang: "Integrated regulation of AMPA glutamate receptor phosphorylation in the striatum by dopamine and acetylcholine." in: Neuropharmacology, (2016) (PubMed).

Pasek, Wang, Colbran: "Differential CaMKII regulation by voltage-gated calcium channels in the striatum." in: Molecular and cellular neurosciences, Vol. 68, pp. 234-43, (2016) (PubMed).

Mao, Xue, Jin, Wang: "Dynamic increases in AMPA receptor phosphorylation in the rat hippocampus in response to amphetamine." in: Journal of neurochemistry, Vol. 133, Issue 6, pp. 795-805, (2015) (PubMed).

Mao, Hastings, Fibuch, Wang: "Propofol selectively alters GluA1 AMPA receptor phosphorylation in the hippocampus but not prefrontal cortex in young and aged mice." in: European journal of pharmacology, Vol. 738, pp. 237-44, (2014) (PubMed).

Xue, Edwards, Mao, Guo, Jin, Fibuch, Wang: "Rapid and sustained GluA1 S845 phosphorylation in synaptic and extrasynaptic locations in the rat forebrain following amphetamine administration." in: Neurochemistry international, Vol. 64, pp. 48-54, (2013) (PubMed).

Ferrario, Loweth, Milovanovic, Wang, Wolf: "Distribution of AMPA receptor subunits and TARPs in synaptic and extrasynaptic membranes of the adult rat nucleus accumbens." in: Neuroscience letters, Vol. 490, Issue 3, pp. 180-4, (2011) (PubMed).

Jiao, Jalan-Sakrikar, Robison, Baucum, Bass, Colbran: "Characterization of a central Ca2+/calmodulin-dependent protein kinase IIalpha/beta binding domain in densin that selectively modulates glutamate receptor subunit phosphorylation." in: The Journal of biological chemistry, Vol. 286, Issue 28, pp. 24806-18, (2011) (PubMed).

Sears, Liu, Narayanan, Sharf, Yeckel, Laubach, Aghajanian, DiLeone: "Regulation of nucleus accumbens activity by the hypothalamic neuropeptide melanin-concentrating hormone." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 24, pp. 8263-73, (2010) (PubMed).

Target Details for Glutamate Receptor 1

Target: Glutamate Receptor 1 (GLUR1)

Alternative Name: GRIA1

Background:

Synonyms: GLUR 1 antibody, GLUR A antibody, AMPA 1 antibody, AMPA selective glutamate receptor 1 antibody, AMPA-selective glutamate receptor 1 antibody, GluA1 antibody, GLUH1 antibody, GluR K1 antibody, GluR-1 antibody, GluR-A antibody, GluR-K1 antibody, GLUR1 antibody, GLURA antibody, GluRK1 antibody, Glutamate receptor 1 antibody, Glutamate receptor ionotropic AMPA 1 antibody, Glutamate receptor ionotropic antibody, Glutamate receptor, ionotropic, AMPA 1 antibody, Gria1 antibody, GRIA1_HUMAN antibody, HBGR1 antibody, MGC133252 antibody, OTTHUMP00000160643 antibody, OTTHUMP00000165781 antibody, THUMP00000224241 antibody, OTTHUMP00000224242 antibody, OTTHUMP00000224243 antibody

Description Logic: Our GluR1-Subunit (Ser831) rabbit polyclonal phosphospecific primary antibody is produced . It detects GluR1-Subunit (Ser831) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Gene Name Alternatives: GRIA1

Molecular Weight: 100 kDa

Gene ID: 50592

NCBI Accession: NP_113796

UniProt: P19490

Pathways: PI3K-Akt Signaling