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Synaptotagmin antibody (pThr202)

The Rabbit Polyclonal anti-Synaptotagmin antibody has been validated for WB, ELISA and ICC. It is suitable to detect Synaptotagmin in samples from Rat.
Catalog No. ABIN361496

Quick Overview for Synaptotagmin antibody (pThr202) (ABIN361496)

Target

See all Synaptotagmin (SYT) Antibodies
Synaptotagmin (SYT)

Reactivity

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Rat

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This Synaptotagmin antibody is un-conjugated

Application

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Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
  • Binding Specificity

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    pThr202

    Specificity

    Specific for endogenous levels of the ~62 kDa synaptotagmin protein phosphorylated at Thr 202. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

    Cross-Reactivity

    Rat

    Predicted Reactivity

    bovine, canine, chicken, human, mouse, non-human primate, zebra fish

    Characteristics

    Our Synaptotagmin (Thr202) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects Synaptotagmin (Thr202) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, ICC.

    Purification

    Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

    Immunogen

    Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202 of rat synaptotagmin, conjugated to keyhole limpet hemocyanin (KLH).

    Isotype

    IgG
  • Application Notes

    Dilution Range: ICC: 1:400

    Dilution Range: WB Brain: 1:1000

    Dilution Range: WB: 1:1000

    Restrictions

    For Research Use only
  • Format

    Liquid

    Buffer

    10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

    Storage

    -20 °C

    Storage Comment

    Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

    Expiry Date

    12 months
  • Target

    Synaptotagmin (SYT)

    Alternative Name

    Synaptotagmin

    Background

    Synonyms: DKFZp781D2042 antibody, FLJ42519 antibody, P65 antibody, SVP65 antibody, synaptotagmin 1 antibody, Synaptotagmin I antibody, SYT antibody, SYT1 antibody, SytI antibody

    Description Logic: Our Synaptotagmin (Thr202) rabbit polyclonal phosphospecific primary antibody is produced . It detects Synaptotagmin (Thr202) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, ICC.

    Gene Name Alternatives: SYT1

    Molecular Weight

    62 kDa

    Gene ID

    25716

    UniProt

    P21707
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