Synaptotagmin antibody (pSer309)

Catalog No. ABIN6256263

Product Details anti-Synaptotagmin Antibody

Target: Synaptotagmin (SYT)

Binding Specificity: pSer309

Reactivity: Rat, Mouse, Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Synaptotagmin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Phospho-Synaptotagmin (Ser309) Antibody detects endogenous levels of Synaptotagmin only when phosphorylated at Serine 309

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human Synaptotagmin around the phosphorylation site of Serine 309

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000 IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

Target Details for Synaptotagmin

Target: Synaptotagmin (SYT)

Alternative Name: Synaptotagmin (SYT Products)

Synonyms: Syti antibody, GB20036 antibody, DKFZP459P193 antibody, SYT1 antibody, SytI antibody, SyNapTotagmin antibody, synaptotagmin antibody, synaptotagmin 1 antibody, snt-5 antibody, snt-6 antibody, snt-3 antibody, Syt antibody, Syt1 antibody, SYT1 antibody

Background:

Description: May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca2+-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca2+-independent manner, these are neurexins, syntaxin and AP2. Plays a role in dendrite formation by melanocytes (PubMed:23999003).

Gene: SYT1

Molecular Weight: 47kDa

Gene ID: 6857

UniProt: P21579, Q8N9I0