Synaptotagmin antibody

Catalog No. ABIN7217178

The Rabbit Polyclonal anti-Synaptotagmin antibody is suitable to detect Synaptotagmin in samples from Rat, Mouse and Human. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for Synaptotagmin antibody (ABIN7217178)

Target: Synaptotagmin (SYT)

Reactivity: Rat, Mouse, Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Synaptotagmin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Synaptotagmin Antibody

Purpose: Rabbit Anti-Synaptotagmin Polyclonal Antibody

Specificity: Synaptotagmin Polyclonal Antibody detects endogenous levels of Synaptotagmin protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Synaptotagmin 1/2 around the non-phosphorylation site of T202/199

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Synaptotagmin

Target: Synaptotagmin (SYT)

Alternative Name: Synaptotagmin

Background: SYT1, SVP65, SYT, Synaptotagmin-1, Synaptotagmin I, SytI, p65, SYT2, Synaptotagmin-2, Synaptotagmin II, SytIIThe synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse.

Molecular Weight: 60kD

Gene ID: 6857, 127833

UniProt: P21579, Q8N9I0