Synaptotagmin antibody (pSer309)

Catalog No. ABIN361497

This anti-Synaptotagmin antibody is a Rabbit Polyclonal antibody detecting Synaptotagmin in WB, ELISA and ICC. Suitable for Rat. This Primary Antibody has been cited in 5+ publications.

Quick Overview for Synaptotagmin antibody (pSer309) (ABIN361497)

Target: Synaptotagmin (SYT)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Synaptotagmin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Product Details anti-Synaptotagmin Antibody

Binding Specificity: pSer309

Specificity: Specific for endogenous levels of the ~62 kDa synaptotagmin protein phosphorylated at Ser 309. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Cross-Reactivity: Rat

Predicted Reactivity: bovine, canine, chicken, human, mouse, non-human primate, zebra fish

Characteristics: Our Synaptotagmin (Ser309) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects Synaptotagmin (Ser309) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, ICC.

Purification: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser309 of rat synaptotagmin, conjugated to keyhole limpet hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

Dilution Range: ICC: 1:400

Dilution Range: WB Brain: 1:1000

Dilution Range: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Expiry Date: 12 months

References for anti-Synaptotagmin antibody (ABIN361497)

Leal-Ortiz, Waites, Terry-Lorenzo, Zamorano, Gundelfinger, Garner: "Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis." in: The Journal of cell biology, Vol. 181, Issue 5, pp. 831-46, (2008) (PubMed).

Poskanzer, Marek, Sweeney, Davis: "Synaptotagmin I is necessary for compensatory synaptic vesicle endocytosis in vivo." in: Nature, Vol. 426, Issue 6966, pp. 559-63, (2003) (PubMed).

Wang, Lu, Bai, Chang, Martin, Chapman, Jackson: "Different domains of synaptotagmin control the choice between kiss-and-run and full fusion." in: Nature, Vol. 424, Issue 6951, pp. 943-7, (2003) (PubMed).

Fernández-Chacón, Königstorfer, Gerber, García, Matos, Stevens, Brose, Rizo, Rosenmund, Südhof: "Synaptotagmin I functions as a calcium regulator of release probability." in: Nature, Vol. 410, Issue 6824, pp. 41-9, (2001) (PubMed).

Hilfiker, Pieribone, Nordstedt, Greengard, Czernik: "Regulation of synaptotagmin I phosphorylation by multiple protein kinases." in: Journal of neurochemistry, Vol. 73, Issue 3, pp. 921-32, (1999) (PubMed).

Target Details for Synaptotagmin

Target: Synaptotagmin (SYT)

Alternative Name: Synaptotagmin

Background:

Synonyms: DKFZp781D2042 antibody, FLJ42519 antibody, P65 antibody, SVP65 antibody, synaptotagmin 1 antibody, Synaptotagmin I antibody, SYT antibody, SYT1 antibody, SytI antibody

Description Logic: Our Synaptotagmin (Ser309) rabbit polyclonal phosphospecific primary antibody is produced . It detects Synaptotagmin (Ser309) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, ICC.

Gene Name Alternatives: SYT1

Molecular Weight: 60 kDa/62 kDa

Gene ID: 25716

UniProt: P21707