Tyrosine Hydroxylase antibody (pSer31)

Catalog No. ABIN361502

The Rabbit Polyclonal anti-Tyrosine Hydroxylase antibody has been validated for WB, IHC, ICC and ELISA. It is suitable to detect Tyrosine Hydroxylase in samples from Rat. There are 5+ publications available.

Quick Overview for Tyrosine Hydroxylase antibody (pSer31) (ABIN361502)

Target: Tyrosine Hydroxylase (TH)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tyrosine Hydroxylase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), ELISA

Product Details anti-Tyrosine Hydroxylase Antibody

Binding Specificity: pSer31

Specificity: Specific for endogenous levels of the ~60 kDa tyrosine hydroxylase protein phosphorylated at Ser31.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: non-human primate

Characteristics: Our Tyrosine Hydroxylase (Ser31) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects Tyrosine Hydroxylase (Ser31) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, IHC, ICC.

Purification: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser31 of rat tyrosine hydroxylase, conjugated to keyhole limpet hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

Dilution Range: ICC: 1:50-1:300

Dilution Range: WB Brain: 1:1000

Dilution Range: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Expiry Date: 12 months

References for anti-Tyrosine Hydroxylase antibody (ABIN361502)

Calipari, Ferris, Melchior, Bermejo, Salahpour, Roberts, Jones: "Methylphenidate and cocaine self-administration produce distinct dopamine terminal alterations." in: Addiction biology, Vol. 19, Issue 2, pp. 145-55, (2014) (PubMed).

Izumi, Ezumi, Takada-Takatori, Akaike, Kume: "Endogenous dopamine is involved in the herbicide paraquat-induced dopaminergic cell death." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 139, Issue 2, pp. 466-78, (2014) (PubMed).

Nakashima, Mori, Kaneko, Hayashi, Nagatsu, Ota: "Phosphorylation of the N-terminal portion of tyrosine hydroxylase triggers proteasomal digestion of the enzyme." in: Biochemical and biophysical research communications, Vol. 407, Issue 2, pp. 343-7, (2011) (PubMed).

Jedynak, Ali, Haycock, Hope: "Acute administration of cocaine regulates the phosphorylation of serine-19, -31 and -40 in tyrosine hydroxylase." in: Journal of neurochemistry, Vol. 82, Issue 2, pp. 382-8, (2002) (PubMed).

Salvatore, Waymire, Haycock: "Depolarization-stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ." in: Journal of neurochemistry, Vol. 79, Issue 2, pp. 349-60, (2001) (PubMed).

Target Details for Tyrosine Hydroxylase

Target: Tyrosine Hydroxylase (TH)

Alternative Name: Tyrosine Hydroxylase

Background:

Synonyms: Dystonia 14 antibody, DYT14 antibody, DYT5b antibody, EC 1.14.16.2 antibody, OTTHUMP00000011225 antibody, OTTHUMP00000011226 antibody, ple antibody, Protein Pale antibody, TH antibody, The antibody, TY3H_HUMAN antibody, TYH antibody, Tyrosine 3 hydroxylase antibody, Tyrosine 3 monooxygenase antibody, Tyrosine 3-hydroxylase antibody, Tyrosine 3-monooxygenase antibody, Tyrosine hydroxylase antibody

Description Logic: Our Tyrosine Hydroxylase (Ser31) rabbit polyclonal phosphospecific primary antibody is produced . It detects Tyrosine Hydroxylase (Ser31) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, IHC, ICC.

Gene Name Alternatives: TH

Molecular Weight: 60 kDa

Gene ID: 25085

UniProt: P04177

Pathways: Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour