KCNQ4 antibody (AA 2-77)

Catalog No. ABIN361768

This Mouse Monoclonal antibody specifically detects KCNQ4 in WB, IHC, IP, ICC, IF and AA. It exhibits reactivity toward Human. It has been mentioned in 3+ publications

Quick Overview for KCNQ4 antibody (AA 2-77) (ABIN361768)

Target: KCNQ4 (Potassium Voltage-Gated Channel, KQT-Like Subfamily, Member 4 (KCNQ4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KCNQ4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Antibody Array (AA)

Clone: N43-6 (Formerly S43-6)

Product Details anti-KCNQ4 Antibody

Binding Specificity: AA 2-77

Specificity: Detects ~77 kDa.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 2-77 of human KCNQ4

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN361768 was sufficient for detection of KCNQ4 in 10 μg of COS-1 cell lysate transiently expressing KCNQ4 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

References for anti-KCNQ4 antibody (ABIN361768)

Marchetta, Möhrle, Eckert, Reimann, Wolter, Tolone, Lang, Wolters, Feil, Engel, Paquet-Durand, Kuhn, Knipper, Rüttiger: "Guanylyl Cyclase A/cGMP Signaling Slows Hidden, Age- and Acoustic Trauma-Induced Hearing Loss." in: Frontiers in aging neuroscience, Vol. 12, pp. 83, (2020) (PubMed).

Jeng, Ceriani, Hendry, Johnson, Yen, Simmons, Kros, Marcotti: "Hair cell maturation is differentially regulated along the tonotopic axis of the mammalian cochlea." in: The Journal of physiology, Vol. 598, Issue 1, pp. 151-170, (2020) (PubMed).

Wolter, Möhrle, Schmidt, Pfeiffer, Zelle, Eckert, Krämer, Feil, Pilz, Knipper, Rüttiger: "GC-B Deficient Mice With Axon Bifurcation Loss Exhibit Compromised Auditory Processing." in: Frontiers in neural circuits, Vol. 12, pp. 65, (2019) (PubMed).

Target Details for KCNQ4

Target: KCNQ4 (Potassium Voltage-Gated Channel, KQT-Like Subfamily, Member 4 (KCNQ4))

Alternative Name: KCNQ4

Background: The protein encoded by this gene forms a potassium channel that is thought to play a critical role in the regulation of neuronal excitability (1), particularly in sensory cells of the cochlea (2). The current generated by this channel is inhibited by M1 muscarinic acetylcholine receptors and activated by retigabine, a novel anti-convulsant drug (3).

Gene ID: 9132

NCBI Accession: NP_004691

UniProt: P56696

Pathways: Sensory Perception of Sound