JunD antibody (pSer255)

Catalog No. ABIN361928

This Rabbit Polyclonal antibody specifically detects JunD in WB and IHC. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for JunD antibody (pSer255) (ABIN361928)

Target: JunD (JUND) (Jun D Proto-Oncogene (JUND))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JunD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-JunD Antibody

Binding Specificity: pSer255

Specificity: The antibody detects endogenous level of JunD only when phosphorylated at serine 255.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer255 (G-E-S (p) -P-P) derived from Human JunD. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for JunD

Target: JunD (JUND) (Jun D Proto-Oncogene (JUND))

Alternative Name: JunD

Background: Binds an AP-1 site and upon cotransfection stimulates the activity of a promoter that bears an AP-1 site.

Molecular Weight: 38 kDa

NCBI Accession: NP_005345

UniProt: P17535