JunD antibody (pSer255)

Catalog No. ABIN361927

Product Details anti-JunD Antibody

Target: JunD (JUND) (Jun D Proto-Oncogene (JUND))

Binding Specificity: pSer255

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JunD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: The antibody detects endogenous level of JunD only when phosphorylated at serine 255.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer255 (G-E-S (p) -P-P) derived from Human JunD. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for JunD

Target: JunD (JUND) (Jun D Proto-Oncogene (JUND))

Alternative Name: JunD (JUND Products)

Background: Binds an AP-1 site and upon cotransfection stimulates the activity of a promoter that bears an AP-1 site.

Molecular Weight: 38 kDa

NCBI Accession: NP_005345

UniProt: P17535