Tumor Protein p73 antibody (pTyr99)

Catalog No. ABIN361986

The Rabbit Polyclonal anti-Tumor Protein p73 antibody is suitable to detect Tumor Protein p73 in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for Tumor Protein p73 antibody (pTyr99) (ABIN361986)

Target: Tumor Protein p73 (TP73) (Tumor Protein P73 (TP73))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tumor Protein p73 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Tumor Protein p73 Antibody

Binding Specificity: pTyr99

Specificity: The antibody detects endogenous level of p73 only when phosphorylated at tyrosine99.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pTyr99 (S-P-Y (p) -A-Q) derived from Human P73. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for Tumor Protein p73

Target: Tumor Protein p73 (TP73) (Tumor Protein P73 (TP73))

Alternative Name: p73

Background: This gene encodes tumor protein p73, which is a member of the p53 family of transcription factors involved in cellular responses to stress and development. The family members include p53, p63, and p73 and have high sequence similarity to one another, which allows p63 and p73 to transactivate p53-responsive genes causing cell cycle arrest and apoptosis. The family members can interact with each other in many ways involving direct or indirect protein interactions, resulting in regulation of the same target gene promoters or regulation of each other's promoters. The p73 protein is expressed at very low levels in normal tissues and is differentially expressed in a number of tumors. The p73 gene expresses at least 35 mRNA variants due to the use of alternate promoters, alternate translation initiation sites, and multiple splice variations. Theoretically this can account for 29 different p73 isoforms, however, the biological validity and the full-length nature of most variants have not been determined.

Molecular Weight: 80 kDa

NCBI Accession: NP_001119

UniProt: O15350

Pathways: Regulation of Cell Size, Positive Regulation of Response to DNA Damage Stimulus