tau antibody (pThr205)

Catalog No. ABIN362074

Cited in 4+ publications. The Rabbit Polyclonal anti-tau antibody (ABIN362074) specifically detects tau in WB and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for tau antibody (pThr205) (ABIN362074)

Target: tau (tau Protein)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This tau antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-tau Antibody

Binding Specificity: pThr205

Specificity: The antibody detects endogenous level of Tau only when phosphorylated at threonine 205.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pThr205 (P-G-T (p) -P-G) derived from Human Tau. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-tau antibody (ABIN362074)

Sun, Wei, Xiong, Wang, Xie, Wang, Yang, Wang, Lu, Liu, Wang: "Synaptic released zinc promotes tau hyperphosphorylation by inhibition of protein phosphatase 2A (PP2A)." in: The Journal of biological chemistry, Vol. 287, Issue 14, pp. 11174-82, (2012) (PubMed).

Chen, Xiong, Kong, Qu, Wang, Chen, Wang, Zhu: "Neuroglobin attenuates Alzheimer-like tau hyperphosphorylation by activating Akt signaling." in: Journal of neurochemistry, Vol. 120, Issue 1, pp. 157-64, (2011) (PubMed).

Tan, Cao, Wang, Lv, Wu, Ma: "Tau hyperphosphorylation is associated with memory impairment after exposure to 1.5% isoflurane without temperature maintenance in rats." in: European journal of anaesthesiology, Vol. 27, Issue 9, pp. 835-41, (2010) (PubMed).

Tan, Cao, Wang, Lv, Wu, Ma: "Protective effects of lithium treatment for spatial memory deficits induced by tau hyperphosphorylation in splenectomized rats." in: Clinical and experimental pharmacology & physiology, Vol. 37, Issue 10, pp. 1010-5, (2010) (PubMed).

Target Details for tau

Target: tau (tau Protein)

Alternative Name: Tau

Background: Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Molecular Weight: 78 kDa

UniProt: P10636