tau antibody (pSer404)

Catalog No. ABIN362082

There are 2+ publications for this product available. The Rabbit Polyclonal anti-tau antibody is suitable to detect tau in samples from Human, Mouse and Rat. It has been validated for WB, IHC and IF.

Quick Overview for tau antibody (pSer404) (ABIN362082)

Target: tau (tau Protein)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This tau antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-tau Antibody

Binding Specificity: pSer404

Specificity: The antibody detects endogenous level of Tau only when phosphorylated at serine 404.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer404 (D-T-S (p) -P-R) derived from Human Tau. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-tau antibody (ABIN362082)

Sun, Wei, Xiong, Wang, Xie, Wang, Yang, Wang, Lu, Liu, Wang: "Synaptic released zinc promotes tau hyperphosphorylation by inhibition of protein phosphatase 2A (PP2A)." in: The Journal of biological chemistry, Vol. 287, Issue 14, pp. 11174-82, (2012) (PubMed).

Chen, Xiong, Kong, Qu, Wang, Chen, Wang, Zhu: "Neuroglobin attenuates Alzheimer-like tau hyperphosphorylation by activating Akt signaling." in: Journal of neurochemistry, Vol. 120, Issue 1, pp. 157-64, (2011) (PubMed).

Target Details for tau

Target: tau (tau Protein)

Alternative Name: Tau

Background: Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Molecular Weight: 78 kDa

NCBI Accession: NP_001116

UniProt: P10636