MYL2 antibody (pSer18)

Catalog No. ABIN362086

The Rabbit Polyclonal anti-MYL2 antibody (ABIN362086) specifically detects MYL2 in IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for MYL2 antibody (pSer18) (ABIN362086)

Target: MYL2 (Myosin, Light Chain 2, Regulatory, Cardiac, Slow (MYL2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYL2 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-MYL2 Antibody

Binding Specificity: pSer18

Specificity: The antibody detects endogenous level of Myosin Light Chain 2 only when phosphorylated at serine 18.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer18 (A-T-S (p) -N-V) derived from Human Myosin Light Chain 2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for MYL2

Target: MYL2 (Myosin, Light Chain 2, Regulatory, Cardiac, Slow (MYL2))

Alternative Name: Myosin Light Chain 2

Background: Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion

Molecular Weight: 18 kDa

NCBI Accession: NP_006462

UniProt: P19105

Pathways: Myometrial Relaxation and Contraction