Histone H3.1 antibody (pSer10)

Catalog No. ABIN362204

This Rabbit Polyclonal antibody specifically detects Histone H3.1 in WB, IF and IHC. It exhibits reactivity toward Human, Mouse and Rat. It has been mentioned in 4+ publications

Quick Overview for Histone H3.1 antibody (pSer10) (ABIN362204)

Target: Histone H3.1 (HIST1H3B)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H3.1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-Histone H3.1 Antibody

Binding Specificity: pSer10

Specificity: The antibody detects endogenous level of Histone H3.1 only when phosphorylated at serine 10.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer10 (R-K-S (p) -T-G) derived from Human Histone H3.1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-Histone H3.1 antibody (ABIN362204)

Jiang, Li, Yang, Hawke, Zheng, Xia, Aldape, Wei, Guo, Chen, Lu: "PKM2 regulates chromosome segregation and mitosis progression of tumor cells." in: Molecular cell, Vol. 53, Issue 1, pp. 75-87, (2014) (PubMed).

Liao, Dong, Wu, Yang, Wang, Guo, Chen, Xu, Wen: "Resolvin D1 attenuates inflammation in lipopolysaccharide-induced acute lung injury through a process involving the PPAR?/NF-?B pathway." in: Respiratory research, Vol. 13, pp. 110, (2013) (PubMed).

Sun, Chen, Wang, Li, Xue, Qu, Zhang, Mu, Lu: "The protective effect of alpha lipoic acid on Schwann cells exposed to constant or intermittent high glucose." in: Biochemical pharmacology, Vol. 84, Issue 7, pp. 961-73, (2012) (PubMed).

Yang, Xia, Hawke, Li, Liang, Xing, Aldape, Hunter, Alfred Yung, Lu: "PKM2 phosphorylates histone H3 and promotes gene transcription and tumorigenesis." in: Cell, Vol. 150, Issue 4, pp. 685-96, (2012) (PubMed).

Target Details for Histone H3.1

Target: Histone H3.1 (HIST1H3B)

Alternative Name: Histone H3.1

Background: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Molecular Weight: 17 kDa

NCBI Accession: NP_003521

UniProt: P68431