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HSF1 antibody (Heat Shock Factor Protein 1) (pSer307) Primary Antibody

HSF1 Reactivity: Human IF, WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN362220
$306.90
Plus shipping costs $45.00
100 μL
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  • Target
    HSF1
    Binding Specificity
    • 26
    • 15
    • 15
    • 12
    • 11
    • 11
    • 10
    • 10
    • 9
    • 9
    • 8
    • 8
    • 8
    • 7
    • 6
    • 5
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer307
    Reactivity
    • 207
    • 119
    • 89
    • 35
    • 29
    • 29
    • 27
    • 24
    • 7
    • 6
    • 4
    • 1
    • 1
    • 1
    Human
    Host
    • 171
    • 34
    • 9
    • 1
    Rabbit
    Clonality
    • 164
    • 50
    Polyclonal
    Conjugate
    • 107
    • 13
    • 11
    • 10
    • 8
    • 8
    • 7
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This HSF1 antibody is un-conjugated
    Application
    • 163
    • 116
    • 81
    • 64
    • 48
    • 39
    • 32
    • 28
    • 28
    • 28
    • 11
    • 8
    • 6
    • 5
    • 2
    • 1
    • 1
    Immunofluorescence (IF), Western Blotting (WB)
    Specificity
    The antibody detects endogenous level of HSF1 only when phosphorylated at serine 307.
    Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
    Immunogen
    Peptide sequence around phosphorylation site of pSer307 (P-Q-S (p) -P-R) derived from Human HSF1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
    Isotype
    IgG
  • Application Notes
    Western blotting: 1:500-1:1000
    Immunofluorescence: 1:100-1:200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
  • Target
    HSF1
    Alternative Name
    HSF1 (HSF1 Antibody Abstract)
    Synonyms
    hsf1, Hsf, xhsf1, AA960185, HSTF1, HSF1, HSF, HSF1c, hsf1a, hsf1b, zHSF1a, zHSF1b, hstf1, heat shock factor, Heat Shock Factor, heat shock factor 1, heat shock transcription factor 1, heat shock transcription factor 1 S homeolog, hsf1, hsf-1, Hsf1, HSF1, hsf1.S
    Background
    DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
    Molecular Weight
    82 kDa
    NCBI Accession
    NP_005517
    UniProt
    Q00613
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