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beta Catenin antibody (pSer33)

The Rabbit Polyclonal anti-beta Catenin antibody is suitable to detect beta Catenin in samples from Human, Mouse and Rat. It has been validated for WB and IHC.
Catalog No. ABIN362256
$350.90
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Quick Overview for beta Catenin antibody (pSer33) (ABIN362256)

Target

See all beta Catenin (CATNB) Antibodies
beta Catenin (CATNB) (Catenin, beta (CATNB))

Reactivity

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Human, Mouse, Rat

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This beta Catenin antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC)
  • Binding Specificity

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    pSer33

    Specificity

    The antibody detects endogenous level of b-Catenin only when phosphorylated at serine 33.

    Purification

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

    Immunogen

    Peptide sequence around phosphorylation site of pSer33 (L-D-S (p) -G-I) derived from Human b-Catenin. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

    Isotype

    IgG
  • Application Notes

    Western blotting: 1:500-1:1000
    Immunohistochemistry: 1:50-1:100

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C/-20 °C

    Storage Comment

    Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
  • Target

    beta Catenin (CATNB) (Catenin, beta (CATNB))

    Alternative Name

    beta-Catenin

    Background

    Involved in the regulation of cell adhesion and in signal transduction through the Wnt pathway.

    Molecular Weight

    92 kDa

    NCBI Accession

    NP_001091

    UniProt

    P35222

    Pathways

    Peptide Hormone Metabolism
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