ERK1/2 antibody (pTyr204)

Catalog No. ABIN362302

Cited in 1 publication. The Rabbit Polyclonal anti-ERK1/2 antibody (ABIN362302) specifically detects ERK1/2 in WB, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for ERK1/2 antibody (pTyr204) (ABIN362302)

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERK1/2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-ERK1/2 Antibody

Binding Specificity: pTyr204

Specificity: The antibody detects endogenous level of p44/42 MAP Kinase only when phosphorylated at tyrosine 204.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pTyr204 (T-E-Y (p) -V-A) derived from Human p44, 42 MAP Kinase. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-ERK1/2 antibody (ABIN362302)

Gao, Wen, Yang, Lu, Tong, Huang, Liu, Tang: "Celecoxib ameliorates portal hypertension of the cirrhotic rats through the dual inhibitory effects on the intrahepatic fibrosis and angiogenesis." in: PLoS ONE, Vol. 8, Issue 7, pp. e69309, (2013) (PubMed).

Target Details for ERK1/2

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Alternative Name: p44/42 MAP Kinase

Background: Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1, required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2).

Molecular Weight: 44 kDa

NCBI Accession: NP_001035

UniProt: P27361