SAPK, JNK (AA 181-185) antibody
(1 reference)-
- Target
- SAPK, JNK
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Binding Specificity
- AA 181-185
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
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Application
- Immunohistochemistry (IHC), Western Blotting (WB)
- Specificity
- The antibody detects endogenous level of total SAPK/JNK protein.
- Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Immunogen
- Peptide sequence around AA 181-185 (M-M-T-P-Y) derived from Human SAPK, JNK. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
- Isotype
- IgG
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anti-SAPK, JNK (pTyr185) antibody
Reactivity: Human, Mouse, Rat IHC, WB, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-SAPK, JNK (pThr183) antibodyReactivity: Human, Mouse, Rat IHC, WB, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-SAPK, JNK (AA 166-215) antibodyReactivity: Human, Mouse, Rat IHC, WB, ELISA, IF Host: Rabbit Polyclonal unconjugated
anti-SAPK, JNK (AA 151-200), (pTyr185) antibodyReactivity: Human, Mouse, Rat IHC, WB, ELISA Host: Rabbit Polyclonal unconjugated
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- Application Notes
-
Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
-
-
: "Activation by phosphorylation and purification of human c-Jun N-terminal kinase (JNK) isoforms in milligram amounts." in: Protein expression and purification, Vol. 75, Issue 2, pp. 138-46, (2010) (PubMed).
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: "Activation by phosphorylation and purification of human c-Jun N-terminal kinase (JNK) isoforms in milligram amounts." in: Protein expression and purification, Vol. 75, Issue 2, pp. 138-46, (2010) (PubMed).
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- Target
- SAPK, JNK
- Alternative Name
- SAPK/JNK
- Synonyms
- JNK-55 antibody, JNK2 antibody, JNK2A antibody, JNK2ALPHA antibody, JNK2B antibody, JNK2BETA antibody, PRKM9 antibody, SAPK antibody, SAPK1a antibody, p54a antibody, p54aSAPK antibody, AI851083 antibody, Prkm9 antibody, mitogen-activated protein kinase 9 antibody, MAPK9 antibody, Mapk9 antibody
- Background
- Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells.
- Molecular Weight
- 54 kDa
- NCBI Accession
- NP_001128
- UniProt
- P53779
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