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HSF1 antibody (AA 301-305)

HSF1 Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated

Catalog No. ABIN362942

Datasheet as PDF

Target See all HSF1 Antibodies

HSF1 (Heat Shock Factor Protein 1 (HSF1))
Binding Specificity
17

16

15

12

11

9

9

7

7

6

6

6

6

5

4

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 301-305
Reactivity
140

91

38

14

8

7

7

6

6

4

4

1

1

1
Human
Host
129

39

6

1
Rabbit
Compatible Secondaries
Clonality
120

54
Polyclonal
Conjugate
96

10

10

8

8

6

5

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
This HSF1 antibody is un-conjugated
Application
140

96

74

54

49

38

35

19

15

15

6

5

4

3

3

2

1

1
Western Blotting (WB), Immunohistochemistry (IHC)

Specificity

The antibody detects endogenous level of total HSF1 H3.1 protein.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen

Peptide sequence around AA 301-305 (P-P-S-P-P) derived from Human HSF1. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotype

IgG

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anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Alkaline Phosphatase (AP))
HSF1 Reactivity: Mouse WB, ELISA, IHC, IP, IF, GS, ICC Host: Rat Monoclonal 10H8 Alkaline Phosphatase (AP)

anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (HRP)
HSF1 Reactivity: Mouse WB, ELISA, IHC, IP, IF, GS, ICC Host: Rat Monoclonal 10H8 HRP

anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (FITC)
HSF1 Reactivity: Mouse WB, ELISA, IHC, IP, IF, GS, ICC Host: Rat Monoclonal 10H8 FITC

anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (APC)
HSF1 Reactivity: Mouse WB, ELISA, IHC, IP, IF, GS, ICC Host: Rat Monoclonal 10H8 APC

anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Biotin)
HSF1 Reactivity: Mouse WB, ELISA, IHC, IP, IF, GS, ICC Host: Rat Monoclonal 10H8 Biotin

anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (PE)
HSF1 Reactivity: Mouse WB, ELISA, IHC, IP, IF, GS, ICC Host: Rat Monoclonal 10H8 PE

anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (PerCP)
HSF1 Reactivity: Mouse WB, ELISA, IHC, IP, IF, GS, ICC Host: Rat Monoclonal 10H8 PerCP

anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Atto 488)
HSF1 Reactivity: Mouse WB, ELISA, IHC, IP, IF, GS, ICC Host: Rat Monoclonal 10H8 Atto 488

anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Atto 594)
HSF1 Reactivity: Mouse WB, ELISA, IHC, IP, IF, GS, ICC Host: Rat Monoclonal 10H8 Atto 594

anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Atto 390)
HSF1 Reactivity: Mouse WB, ELISA, IHC, IP, IF, GS, ICC Host: Rat Monoclonal 10H8 Atto 390

Application Notes

Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C/-20 °C

Storage Comment

Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Target

HSF1 (Heat Shock Factor Protein 1 (HSF1))

Alternative Name

HSF1 (HSF1 Products)

Background

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

Molecular Weight

82 kDa

NCBI Accession

NP_005517

UniProt

Q00613