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H2AFX antibody (H2A Histone Family, Member X) (AA 137-141) Primary Antibody

H2AFX Reactivity: Human IF, IHC, WB Host: Rabbit Polyclonal unconjugated
Pubmed (5)
Catalog No. ABIN362952
$306.90
Plus shipping costs $45.00
100 μL ABIN362952
100 μL ABIN362952
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Delivery in 6 to 8 Business Days
  • Target
    H2AFX
    Binding Specificity
    • 34
    • 27
    • 16
    • 15
    • 12
    • 7
    • 7
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 137-141
    Reactivity
    • 160
    • 87
    • 67
    • 18
    • 13
    • 12
    • 12
    • 8
    • 6
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Human
    Host
    • 144
    • 18
    • 1
    Rabbit
    Clonality
    • 121
    • 42
    Polyclonal
    Conjugate
    • 108
    • 9
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This H2AFX antibody is un-conjugated
    Application
    • 120
    • 52
    • 52
    • 36
    • 31
    • 16
    • 16
    • 9
    • 9
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
    Specificity
    The antibody detects endogenous level of total Histone H2A.X protein.
    Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.
    Immunogen
    Peptide sequence around AA 137-141 (Q-A-S-Q-E) derived from Human Histone H2A.X. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
    Isotype
    IgG
  • Application Notes
    Western blotting: 1:500-1:1000
    Immunohistochemistry: 1:50-1:100
    Immunofluorescence: 1:100-1:200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
  • Qing, Liang, Du, Fan, Xu, Xu, Shi: "Apoptosis induced by trimethyltin chloride in human neuroblastoma cells SY5Y is regulated by a balance and cross-talk between NF-κB and MAPKs signaling pathways." in: Archives of toxicology, Vol. 87, Issue 7, pp. 1273-85, 2013 (PubMed).

    Li, Hu, He, Li, Zhu, Hou, Zhang, Li, Zhang, Wang, Liu, Yao, Zhang: "Up-regulation of NDRG2 through nuclear factor-kappa B is required for Leydig cell apoptosis in both human and murine infertile testes." in: Biochimica et biophysica acta, Vol. 1822, Issue 2, pp. 301-13, 2012 (PubMed).

    Li, Kikuchi, Zheng, Noguchi, Takahashi, Nishida, Masuda, Yang, Takano: "Downregulation and translocation of nuclear ING4 is correlated with tumorigenesis and progression of head and neck squamous cell carcinoma." in: Oral oncology, Vol. 47, Issue 3, pp. 217-23, 2011 (PubMed).

    Li, Nishida, Noguchi, Zheng, Takahashi, Yang, Masuda, Takano: "Decreased nuclear expression and increased cytoplasmic expression of ING5 may be linked to tumorigenesis and progression in human head and neck squamous cell carcinoma." in: Journal of cancer research and clinical oncology, Vol. 136, Issue 10, pp. 1573-83, 2010 (PubMed).

    Dickey, Redon, Nakamura, Baird, Sedelnikova, Bonner: "H2AX: functional roles and potential applications." in: Chromosoma, Vol. 118, Issue 6, pp. 683-92, 2009 (PubMed).

  • Target
    H2AFX
    Alternative Name
    Histone H2A.X (H2AFX Antibody Abstract)
    Synonyms
    H2A.X, H2A/X, H2AX, AW228881, H2ax, Hist5-2ax, gammaH2ax, zgc:56329, h2a.x, h2a/x, h2ax, RGD1566119, h2a, h2afx, H2A histone family member X, H2A histone family, member X, H2A histone family member X L homeolog, histone cluster 1, H2ah, histone cluster 2, H2ab S homeolog, histone H2AX, H2AFX, H2afx, h2afx, h2afx.L, HIST1H2AH, hist2h2ab.S, LOC100522201, LOC100720536
    Background
    Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation
    Molecular Weight
    15 kDa
    NCBI Accession
    NP_002096
    UniProt
    P16104
    Pathways
    Telomere Maintenance, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus
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